Sentence examples for binding to the clamp from inspiring English sources

Therefore, the A subunit binds two clamp subunits, with the A domain binding to the clamp I subunit and the A' domain binding to the clamp III subunit.

The elastic model was further used to construct the energy landscape of the opened PCNA clamp, including conformations that would allow binding to the clamp loader and loading onto double-stranded DNA.

The structure explains the formation of a unique heterotrimeric arrangement and reveals significant differences among the three subunits in the sites implicated in binding to the clamp loader and to ligand proteins.

5) The authors showed that deletions of the TFEα ZR removes binding to the clamp and a deletion of the TFEβ WH weakens PIC formation.

To increase binding to the clamp, amino acid residues 920 924 of E. coli PolIIIα were changed by site directed mutagenesis from QADMF to QLDLF, while in the exonuclease residues 182 187 were changed from QTSMAF to QLSLPL, based on sequences described in (Wijffels et al., 2004).

In addition, Pol IV binds to the clamp using the canonical binding motif (Bunting et al, 2003).

In contrast, the mutant exonuclease185/187 has lost all affinity for the clamp and travels unaltered at all three concentrations, providing proof that the exonuclease binds to the clamp using a canonical binding motif.

Yet our cryo-EM models show that the OB domain is ∼40 Å away from the ssDNA and that it is involved in binding to the sliding clamp.

The processivity of Pol III is greatly enhanced upon binding to the DNA sliding clamp (β subunit) a ring-shaped molecule that encircles the DNA (LaDuca et al, 1986; Stukenberg et al, 1991).

In particular, TnsE promotes preferential insertion into transferred conjugal DNA using two critical features: DNA structure and specific binding to the sliding β-clamp [25], [34].

The N- and C-termini of EGF are not responsible for high-affinity binding to the receptor, so placement of the claMP Tag on either terminus would not be expected to diminish EGF activity, and indeed it does not.