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Efficiency of concatemers binding to the cells is improved with increase of the length and concentration of concatemeric complexes.
FK 33-824 alsuppressedsed PKC activity reducing [3H]PDBu specific binding to theca cells, whereas ionomycin (a positive control) increased labeled phorbol ester binding to the cells.
In contrast, up to 1.7×10−5 M of shikonin failed to inhibit stromal cell-derived factor-1 (SDF-1α) binding to the cells.
To develop the assays, she needed two resources not available at Oberlin: specific ovarian cancer cell lines and a flow cytometer to test binding to the cells.
The researchers also found that the antibodies decreased angiostatin binding to the cells by more than half, which in turn led to an 80% decrease in angiostatin's ability to inhibit endothelial cell growth.
The extent of TA binding to the cells was assessed by fluorescence microscopic analysis (TE2000-E, Nikon, final magnification 60x).
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In B. subtilis, ComGA is absolutely required for initial DNA binding to the cell surface as comGA point mutants are unable to bind to extracellular DNA 66.
PIP3 then binds to the pleckstrin homology domain of Akt/PKB, allowing for its translocation and binding to the cell membrane.
All bacteria used prevented VSV binding to the cell monolayers by direct binding of VSV to their surface.
The transformed yeasts after cell wall digestion showed a green fluorescence derived from fluorescent antibodies binding to the cell membrane, while other cells showed no fluorescence.
Most importantly, Fab-decorated NP binding to the cell surface receptor was monitored by fluorescence-activated cell sorting.
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