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Experiments on the negative control target (EGFR) revealed a reduced binding to the background level (p<0.05) (Figure 1).
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Only oligonucleotides capable of binding to the target or background materials necessary for carrying out the experiment are enriched during that process [ 14].
By contrast, F207A was wild-type (WT -like in its ability to bind liposomes, WT -like40A/D144A showed sinnitscabilityeduced liposome binding close to the bindground liposomesbinding seen in a ΔC2 construct.
4) Figure 3A and B. The binding of TTR to the rc and the β peptides is explained by the background binding to the column material.
The data was processed by subtracting background binding to the reference flow channels (FC1 & FC3).
No protein was injected into FC1, in order to serve as control for background binding to the dextran matrix.
The S10 extract was then complemented with 200 µg/mL of an RNA poly-C homopolymer (GE Lifesciences) to adsorb nonspecific binding proteins and reduce background binding to the RNA affinity column.
The binding of CD58-BV was dependent on CD2 because only minimal background binding to the wells coated with wild type BV was detected (Fig. 2B left panel).
SPR data were corrected for background binding to the matrix of the chip ("blank" channel) and analyzed with BIAevaluation 4.1 software (Biacore, Piscataway, NJ).
The significant number of potential lead sequences allowed for the application of more stringent lead criteria such as good solubility, high peptide sample purity and low background binding to the SPR chip surface.
Both Def11 500 and Def11 500/CUEm bound to such beads, but showed only background binding to the empty control beads.
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