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The characteristic of the complexes obtained by serial dilutions of wild-type phiC31 integrase binding to the attachment sites in bandshift experiments was considered at first.
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The episomal replication is due to stable association with early replication foci by MARS mediated binding to the nuclear matrix protein scaffold attachment factor A (SAF-A).
The interaction with the GHS receptor might either depend on ghrelin binding to membrane in order to more easily find its target or the binding to the receptor could involve simultaneous attachment of ghrelin to the membrane.
Recently we isolated and identified palmitic acid (PA) as a novel natural small molecule that inhibits HIV-1 fusion and infection by the mechanism of binding to the CD4 receptor and blocking gp120-to-CD4 gp120-to-CD4 gp120-to-CD4
The exposed D region of pseudomonal pilin is proposed to mediate attachment to epithelia through binding to the glycolipids asialo-GM1 and asialo-GM2 (Craig et al., 2004).
SICAM-1 is capable of inhibiting lymphocyte attachment to endothelial cells by binding to the ICAM ligands on circulating cells [ 44].
Afa/Dr adhesins mediate bacterial attachment onto target cells by binding to the complement regulatory glycosylphophatidylinositol- GPI- anchored protein DAF [ 166].
The present study may provide a first step in identifying target proteins for such drugs, implicating weak biophysical forces rather then covalent attachments and binding to broadly distributed chromosome bio-molecules rather than specific binding to the telosome nucleoprotein complex.
The recombination pathway begins with binding of integrase to the attachment sites, which are then brought together by protein:protein interactions to form a synaptic tetramer.
The main properties are the binding to: 1- the processing-attachment site at the LTR (long terminal repeat) ends of virus DNA with a Kd (dissociation constant) in the sub-micromolar range; 2- the whole IN enzyme; and 3- the IN binding domain (IBD) but not the IBD-Asp366Asn variant of LEDGF (lens epidermal derived growth factor) lacking the essential Asp366 residue.
SAF-B has been considered a major component of the nuclear matrix, determined by its binding to Scaffold/Matrix Attachment Region DNAs.
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