Sentence examples for binding to the aptamer from inspiring English sources
A panel of ovarian cancer cell lines was tested for binding with aptamer TOV6: serous(serous), C13 and TOV-112D (both endometroid), and OVCAR3 (serous) showed binding to the aptamer, while the aptamer failed to bind other ovarian cell lines, such as OVCAR8 (serous) and A2780 cP (undifferentiated) (Supplemental Table 2, Supporting Information).
Ligand binding to the aptamer prevented pseudoknot formation, thus allowing the RNA-OUT to fold properly and repress its target.
Theophylline binding to the aptamer domain triggers a conformational change in the communication domain, causing a one-nucleotide helix slipping that is sufficient to restore ribosomal accessibility.
The use of a nanometal surface energy transfer probe method for monitoring ATP binding to the aptamer sequence allows the observation of negative cooperativity between the two ATP binding events.
By cross-linking the aptamer to its target, the available protein for analysis is maximized by increasing the solubility, thereby enabling mass spectral techniques to identify the protein binding to the aptamer.
Upon ATX binding to the immobilized aptamer, a significant decrease in the electron-transfer resistance was observed as a result of the aptamer conformation change, which is used as the sensor signal.
We demonstrate that ligand binding to the integrated aptamer domain is associated with a structural change sufficient to inhibit Rnt1p processing.
By targeting specific regions of the signalling functional nucleic acid, the SEO binding to the unbound aptamer triggered perturbations of both the internal DNA flexibility and the localized dye environment upon the free probe to duplex structure transition.
The dissociation constant Kd was determined to be ∼54 nM for the BADGE nanoprobes, in comparison to 10 nM reported for free BPA binding to the same aptamer.
To analyze ligand binding to the Vc2 aptamer, we constructed a 96-plex 20%T3.3%3% C fsPAG for high-throughput riboswitch fsPAG-EMSAs.
Additionally, the Kd value for free BPA binding to the BPA aptamer was determined experimentally using MST to be ∼100 nM (Supporting Information Figure S-1), further validating the usefulness of the capture nanoprobe for future competitive binding assay development.
