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Ovalbumin-linked haptens were coated on the bottom of microplate to compete with the triazophos in the sample for binding to the antibodies on the AuNP probes.
Alpha-MSH in the samples competed with 125I-labelled α-MSH in binding to the antibodies.
Site directed mutagenesis of TcTS Tyr119 and Trp312 residues, which are implicated in the enzymatic mechanism of trans-sialidase activity, decreases about ten times their binding to the antibodies (Table 2), while the addition of DANA increases their binding to TcTS (Figure 4).
The synthetic methoxy-MA subclass had the highest binding to the antibodies of the four synthetic subclasses tested, followed by hydroxy-, keto- and alpha-MA (Figs. 4 7).
For instance, S. Livingstone and S. Worthington were not easily detected by some of the ELISA methods used, probably because of poor binding to the antibodies.
As controls, no binding to the antibodies or two other multi-subunit complexes of the human Pol II core transcription machinery, TFIIH and Pol II, was observed in counter screenings.
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Bacterial binding to the antibody on the sensor surface changes the resonance parameters, and these changes were quantified as a shift in the sensor's resonance frequency.
A control selection was performed to control for non-specific binding to the antibody-bead matrix.
In contrast to the structural differences, alanine-scanning mutagenesis of BMPR-IA showed that the functional determinants for binding to the antibody and BMP-2 are almost identical.
Antigen modulation refers to antigen endocytosis/modulation after binding to the antibody.
The NPY in standards and samples competed with I-labeled NPY in binding to the antibody.
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