Sentence examples for binding to the ancestral from inspiring English sources
After duplication of the ancestral SR, three mutations in one copy radically weakened binding to the ancestral estrogen response element (ERE) and improved binding to a new set of DNA sequences (steroid response elements, SREs).
The glu25GLY mutation produces a high-affinity but extremely promiscuous TF that retains strong binding to the ancestral ERE while gaining high-affinity binding to 13 novel REs, including the SREs.
Here, K D is the equilibrium dissociation constant (expressed in the units of mol per liter; M) of the TBP binding to the ancestral or minor allele of the promoter under study.
Along both pathways, promiscuous intermediate genotypes gained recognition of the novel SREs, followed by further replacements that eliminated high-affinity binding to the ancestral RE and any transiently acquired targets.
Experimental proof against the proposal by Wolf and Koonin [ 1] that the ancestral small subunit RNA rather than proto-mRNA was involved in stabilizing tRNA binding to the ancestral peptidyl transferase would not appear necessary, due to experimental evidence demonstrating that, on the contemporary ribosome at least, the small subunit only binds tRNA at the P site in the presence of mRNA [ 22].
Taken together, our findings indicate that all direct paths from AncSR1 to AncSR1+RH involve one of two scenarios: losing high-affinity binding to the ancestral and all other REs followed by a gain of binding to new targets, or gaining very promiscuous high-affinity binding followed by a dramatic narrowing of targets.
There is an epistatic interaction between the two 'T' alleles such that separately, they decrease MSL complex binding relative to the ancestral allele, but together in the TT haplotype, they increase MSL complex binding (Wilcoxon Test p = 0.028 for both comparisons [GT vs TT and TA vs TT]).
However, when combined, the TT haplotype results in significantly increased levels of MSL complex binding, relative to the ancestral GA haplotype (Wilcoxon Test p = 0.0289; Figure 2B).
Although in these examples single site binding to the 50S subunit is highlighted, we would suggest that such binding was possible originally with pairs of tRNAs positioned in the adjacent A and P sites, thereby enhancing both the binding of the pair to the ancestral peptidyl transferase and the rate of peptide synthesis.
Interestingly, each T allele, when assayed separately, has a negative effect on MSL binding levels compared to the ancestral G or A allele.
