Suggestions(2)
Exact(2)
It is expected that the sites in the E1 and E2 proteins of HCV for binding to the above cofactors or putative HCV coreceptors may serve as targets for development of preventive vaccines against HCV in the near future.
Competitive binding to native EpCAM and binding to the above mentioned EpCAM glycosylation mutants was performed by flow cytometry using a fluorescence-activated cell sorting (FACS Calibur device and the Cellquest pro software (Becton Dickinson, Heidelberg, Germany).
Similar(58)
Similar results were obtained for ANS binding to the samples described above and the results obtained are shown in Fig. S4.
Both routes lead to PIP3 binding to the PD C2 cleft described above and were exploited an equal number of times.
Competition ELISA assays were performed to better quantify the differences of antibody binding to the mutant peptides observed in the above Western blotting experiments.
This observation also agreed very well with the above mentioned ruthenium binding to the N-terminal BRCA1 RING domain protein.
One possibility is binding to the putative ubiquinone binding site mentioned above.
Morphine, as mentioned above, after binding to the μ-opioid receptor, regulates cell cycle progression by stimulating mitogen-activated protein kinase (MAPK /extracellular growth factor (Erk) pathways [ 20].
NF-κB, STAT1, and STAT2 binding to the distal enhancer was confirmed by site-directed ChIP and found to be stronger than binding to the promoter-proximal sites indicated above.
Can directionality of binding help explain the wide range of KDs measured for NES binding to CRM1? 2) Related to the above point, the authors need to give uncertainties on their binding constants (subsection "Structural determinants of the plus versus minus NES", first paragraph and Figure 4).
However, there is a significant difference in interactions between the phosphate groups and the enzyme in the course of nucleotide binding by RT, compared to the above polymerases; while RT stabilizes nucleotide binding by interactions involving the β3 β4 loop, the corresponding structure in polymerases from family A and B is an α helix [10].
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