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Of relevance to this issue, Casciola-Rosen and coworkers [ 45] have shown aCL binding to surface blebs of apoptotic cells, which would be consistent with the clustering of aCL immunostaining in focal surface regions that we detected in apoptotic endothelial cells.
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During apoptosis, caspase-generated CK-18 fragments have been found in cytoplasmic inclusion bodies that are brought to apoptotic surface blebs, most likely before secondary necrosis occurs.
Initiation of assembly involves FN binding to cell-surface receptors.
No significant binding to cell-surface IGFBPs was observed.
The surface blebs are known to form quickly in case of HC stress (Shi et al., 2005; Goodyear et al., 2008) and are a common preparation artifact (Seoane et al., 2001b).
We further test scFv binding to naturally expressed surface hGPC3.
Peptide-induced membrane permeabilization follows binding to the membrane surface.
In addition, the adherent cells changed morphology, from a distended flattened appearance with pseudopods to a fully rounded shape with occasional surface blebs, suggesting a loss of cytoskeletal integrity.
At this stage, morphological changes (e.g., cell surface blebs) on hepatocytes and endothelium - and recruitment of neutrophils to the vascular lumen - become prominent.
Here we used ICAM-3 GFP ICAM-3 GFP ICAM-3 directoy and demonitorte in transfected HeLa cells that ICAM-3 is present within surfand blebs demonstratetosin.
Typical apoptotic alterations were seen in HL-60 cells with surface blebs and nuclear fragmentation (Fig. 2C).
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