Transcription factors (TFs) regulate gene transcription by binding to specific DNA elements such as promoters, enhancers, silencers, insulators and locus control regions.
Additionally, other factors with sequence-specific binding motifs such as MeCP2 might also recruit transcriptional repressor complexes to specific loci and thereby indirectly mediate rapid remethylation at demethylation-resistant CGs [46].
These fusion proteins direct virus integrations to specific loci and/or chromatin marks bound by the chromatin-binding protein.
We found that decreased levels of either RUNX1/3 or ETS1 lead to a decrease of TAL1 genomic binding at all sites tested, which confirms an important role for these transcription factors in targeting TAL1 to specific loci.
Strongly positive values of parameter β in the GDP model describing TF-DNA binding in specific loci were found in all TF libraries (Table 2, Additional file 2).
Thus, mutation of CTCF binding sites at specific loci may dysregulate the expressions of tumor suppression genes or oncogenes, thereby contributing to the malignant phenotype (Filippova et al., 2002).
Naturally, TF-DNA binding avidity could be quantified by the number of TF molecules binding a given specific locus including specific binding element(s) and its flanking regions.
Its regulation takes place through the binding of proteins to DNA at specific loci in the vicinity of the gene to be regulated.
The binding of a TF to a specific locus can be developmental-stage or cell-type specific; alternatively, as growing evidence suggests, sometimes a protein binds to a specific locus constitutively, i.e., in all the cell types studied so far.
Non-specific binding was subtracted from total binding to obtain specific binding.
