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The encounter of a Ca2+ ion with a protein and its subsequent binding to specific binding sites is an intricate process that cannot be fully elucidated from experimental observations.
Since neither of the tail inserts contain any obvious structural motifs nor apparent binding sites they may control the overall conformation of the myosin VI molecule and thus possibly its binding to specific binding partners.
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After phosphorylation, activated VirR regulates the expression of its target genes by binding to specific DNA binding sites [20] via its C-terminal DNA binding domain [21].
Transcription factors (TFs) play important roles in the regulation of gene transcription through binding to specific DNA sequences called TF binding sites (TFBSs), which are usually 5 25 bp in length [1], [2].
To address this issue, we performed gel shift assays measuring global AP-1 binding to specific DNA-binding sites.
The LMO1-encoded protein is suggested to regulate transcription by competitively binding to specific DNA-binding transcription factors.
This could explain why they potentiate the action of oxacillin, which by binding to specific penicillin-binding proteins (PBP) inside the bacterial cell wall inhibits cell wall synthesis.
Secondly, it is difficult to distinguish the contribution of productive (binding to specific glycans) versus non-productive (binding to lignin) binding of cellulases to complex lignocellulosic substrates.
6– 14 TFs regulate gene expression by binding to specific DNA sequences, called transcription factor binding sites (TFBSs), in the promoters of the target genes.
Typically, the SR/CAMTA proteins contain a CG-1 DNA-binding domain (binding to specific cis-elements in promoter regions of the target genes) at the N-terminus, a TIG domain (an immunoglobulin-like fold involved in nonspecific DNA binding), three ankyrin repeats (implicated in protein-protein interaction) and five putative CaM-binding motifs called as IQ motif [ 11- 13].
TFs activate or repress gene transcription by binding to specific sites (transcription factor binding sites, or TFBSs) in promoter regions, thus regulating gene expression at the transcription level; miRNAs inhibit mRNA translation by inducing mRNA degradation and/or blocking the translation machinery, thus negatively regulates gene expression at the post-transcriptional level.
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