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Asc10 expression on the donor cell surface promotes binding to recipient cells.
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Exosome-mimetic NVs delivered Sphk2 to recipient cells in vitro and in vivo.
We found that the NVs could efficiently deliver the specific endogenous molecule Sphk2 to recipient cells.
For each approach, techniques for manipulating HAC in donor cells in order to deliver HAC to recipient cells are required.
We then investigated the ability of ESMVs to transfer GFP to recipient cells.
38 Secretory cell free miRNAs were shown to transfer inhibitory signals to recipient cells.
Linking the role of the uropod and the Rho/mDia1 signaling pathway could be addressed by testing whether inhibition of mDia1 (or RhoA signaling) blocks uropod formation and either the initiation of entosis (i.e., LPAR localization and binding to the recipient cell) or the final stage(s) of engulfment.
Hence we tested BMT recipients for the presence of antidonor antibodies early (1 and 2 weeks) and late (>3 months) after BMT through flow cytometric assessment of recipient serum binding to donor cells.
The recipient cells were then recovered from the filter and plated on LB agar plates containing two antibiotics to select recipient cells that had acquired plasmids.
The selective binding of these NPs was demonstrated with binding to PCNSL cells 3- to 4-fold higher than binding to control cells.
On the right, IT binding to H9/NL4-3 cells is plotted as median fluorescence versus IT concentration.
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