This bond was essential for orientating the peptide into the positively-charged binding pocket on Cbl, to permit binding.
Of particular interest will be to vary the structure of the scorpion ligand to facilitate metal interaction with the DNA helix upon binding, so as to permit the future development of sequence-specific DNA cleavage.
Our finding that hP2X4 (which formed a very low proportion of oligomers in detergent) bound to ATP-coupled beads implies that the presence of a partial binding site may be sufficient to permit ATP binding.
Second, we have included in our tests a randomized probe, which is sufficiently complex to permit binding of ZBTB4, a methyl-binding protein that requires a defined consensus around the methylated CpG [31].
As temperature increases, the hairpin structure can 'melt' and expose the RBS or Shine-Dalgarno sequence to permit binding of the small ribosomal subunit (30S), which then assembles other translation machinery.
HaCaT cells were exposed to AF594-virions for 1 h at 4°C to permit binding but not internalization.
Our results show that even a small subset of these repeats in truncated variants of GvpC proteins is sufficient to permit binding to GVNPs.
The HYR1 and ECE1 genes, which are induced by hypha formation [ 34, 35], contain these consensus sequence motifs, possibly to permit binding of kinase and its downstream target Efg1 to jointly trigger morphogenesis-dependent gene expression.
This premise may apply to the glutamate residues of QacR and, perhaps also, to the multidrug recognition mechanisms of other proteins, which settle for relatively low-affinity binding to their substrates to permit recognition of a myriad of structurally dissimilar ligands.
The transcription factor NF-κB is an example of distinct redox-sensitive activation and DNA binding [57]; it is activated by various physiological stimuli known to produce ROS; on the contrary, to permit DNA binding, similar to Fos, Jun, and Nrf2, cysteine residue within DNA binding domain must be reduced.
