The combinatorial usage of the quasi-independent sites, together with the low sequence specificity of each binding site as well as the structural plasticity of the ANK repeat solenoid (Lee et al., 2006), indicate that ANK repeats can have large capacities in binding to numerous membrane targets with diverse sequences.
Promiscuous binding to several protease targets demonstrates the emerging importance of quantitative studies on binding specificity.
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DOI: http://dx.doi.org/10.7554/eLife.04353.003 The wide-ranging physiological functions of ankyrins are the result of ankyrin's remarkable capacity for binding to and anchoring numerous membrane targets, via their N-terminal 24 ANK repeats, to specific membrane micro-domains in coordination with spectrin-based cytoskeletal structures (Bennett and Chen, 2001).
Eventually, tacrolimus, which was used to inhibit protein phosphatase activity, has complex and numerous effects on the regulation of calcium cycling in the heart through its binding to its cellular target, the tacrolimus binding proteins.
Exploring the chemical space to produce pharmacologically applicable compounds is a daunting task because of an enormous size of the search space and numerous biochemical criteria restricting compound generation, i.e. synthetic feasibility, drug-likeness, and the effective binding to the biological target.
Cyclin A regulates cell cycle progression in association with cyclin-dependent kinases by binding and phosphorylation of numerous target proteins.
Our results suggest that Cul-5 regulates numerous targets relevant to these phenotypes.
