This technology was used to map genome-wide interaction sites of three different lncRNAs including HOTAIR, and has revealed focal, sequence-specific binding at numerous sites in the genome [67].
After induction, RUNX1 binds to numerous de novo sites, initiating a local increase in histone acetylation and rapid global alterations in the binding patterns of SCL/TAL1 and FLI1.
The loss of H3K27me3 observed here could be explained by dilution of a limited amount of PRC2, due to the increased binding of this complex to numerous intergenic sites uncovered by loss of DNA methylation 35.
Many exposures were common to numerous work sites.
HLH-1 binding was also detected at numerous sites unassociated with muscle gene expression, as has been previously described for its mouse homolog MyoD.
These form numerous sites for the binding of Ca2+ ions and are at the origin of the historical name of the protein family, where RTX stands for repeats in toxin (Welch, 2001).
Direct binding studies with K1374R demonstrated no significant alteration in binding to the lower affinity site; however, binding to the high affinity site is reduced by 83%.
Supporting this view, initiator elements do seem to contain numerous binding sites for these early transcription factors and in a few cases, have been shown to be dependent upon the activity of these transcription factors (Busturia and Bienz 1993; Ho et al. 2009; Qian et al. 1991; Shimell et al. 1994; Starr et al. 2011).
For GR DBD binding to κBREs, a two-site binding model was used.
Their HS chains have the ability to bind not only to numerous "heparin-" binding growth factors and morphogens, [ 31] but also to "heparin-" binding sites present in matrix ligands, including fibronectin, vitronectin, laminins, and the fibrillar collagens [ 31].
