Sentence examples for binding to negative targets from inspiring English sources

Negative or counter selections are often performed to increase the specificity of the library or direct the enrichment process away from binding to negative targets.

SPR cross-binding assays were performed to test the specificity of the selected Exotoxin A MRE. Concentrations of all cross-binding analytes were higher than those used in affinity assays to ensure that R14.33 has no nonspecific binding to negative targets.

The MRE showed low cross-binding to negative target bacteria but had similar binding affinity for other members of the Listeria genus.

To determine the cross-binding activity of R14.33 to negative targets, blank selection buffer and 5  μM each of Exotoxin A, BSA, biotin (Sigma; St . Louis MO, USA), Cholera toxin, and streptavidin (Amresco; Solon, OH, USA) were injected into both flow cells with the same conditions as described above.

This suggests that genes, which fail to be repressed by prednisolone in GRdim mice, are either regulated through GR binding to negative GRE elements (nGREs) or indirectly regulated via other GR target genes.

Its emphasis is on increasing stringency in directing binding toward free target of interest and at the same time decreasing binding toward negative targets.

Binding responses showed a much higher affinity of R14.33 to Exotoxin A in solution than to all negative targets (one-way ANOVA: F2,12 = 573.4, P < 0.001), and to streptavidin, a significant component of the immobilization substrate.

Recent reports showed that acetylation of the E. coli response regulator CheY had a negative effect on binding to its targets [47], and that CobB was able to regulate chemotaxis by deacetylation of CheY, shown in vitro and in vivo [48].

It seems to work by binding to multiple targets, she said, which may slow down the development of resistance.

Inhibition of autophagy impaired Zn II -induced reactivation of wtp53 functions, including binding to target promoters and transactivation of target genes, rather enhancing the dominant-negative effects of p53H175.

Promiscuous binding to several protease targets demonstrates the emerging importance of quantitative studies on binding specificity.