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The various CRASPs may play similar roles, with initial interaction of the C-terminus of CFH by CRASP-3 or CRASP-5 followed by binding to internal regions SCR5 to 7 of CFH by CRASP-1 or CRASP-2.
The use of mycoplasma-specific primers binding to internal regions of the 16S rRNA genes may be helpful and result in a higher specificity as was already proposed by others [ 20, 30].
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Backes, S. et al. Tom70 enhances mitochondrial preprotein import efficiency by binding to internal targeting sequences.
A-type lamins also localize to internal regions of the nucleus [4], [5].
To study RIG-I binding to the internal duplex RNA regions, we synthesized a family of structurally well-defined RNA hairpins in which the duplex length was varied, but one terminus was blocked by the presence of a structured, RNA tetraloop.
Confirmation of the mutants was performed by two PCRs (see Fig. S7A in the supplemental material), using primers binding to an internal 300-bp region of the gene (absent in the mutant) and a second PCR using primers binding 300 bp upstream and downstream of the target gene (BPSL2309).
sGAG binding to these regions may compete with other molecules within the crowded environment of a FN fibril19 as all three Hep binding regions have multiple binding partners (see Fig. 1).
By binding to regulatory regions alongside certain genes, SIRT1 helps dictate those patterns.
Each controls the expression of numerous genes by binding to specific regions in the genome.
These probes are designed to hybridize to an internal region of a PCR product.
Translation of hepatitis C virus (HCV) RNA is initiated by cap-independent internal ribosome binding to the 5' noncoding region (NCR).
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