Exact(1)
However, we did observe a tendency of HaloTag-TMR ligand binding to display decreases in fluorescence intensity with increases in protein size (Fig. 3C).
Similar(58)
Further, liver transcriptome analysis revealed 259 mRNAs containing full 8mer-seed miR-122 predicted binding sites to display increased expression following treatment, thus indicating such transcripts to be targeted by endogenous miR-122 in Hepatitis C. It is likely that some of those transcripts play additional therapeutic roles.
(In contrast, we do not expect purely chemical steps such as ATP binding and dissociation to display such a dependence).
To estimate the stoichiometry of the dye binding to phage displaying IQ-tag, a known concentration of phage was incubated with the dye.
This can be relevant in vivo since antibody isotypes impact downstream events through antibody binding to cell displayed Fc receptors [ 20].
The phages with and without bound serum autoantibodies were subsequently isolated with streptavidin-coated magnetic beads, binding to the displayed SBP.
Similarly, the complex of mutant protein R273H (not binding to DNA) displayed almost the same peak H as the free protein.
An in vitro binding system is described to display large full-length proteins on bacteriophage T4 capsid surface at high density.
Perhaps these differences may account for the ability of HupBMtbN to display binding to GC rich DNA.
Later, tools to display binding sites as sequence logos of average information, and sequence walkers showing individual information were incorporated into Delila.
Two genes potentially involved in the recognition of glycosylation, the C-type lectin receptor and the mannose-binding protein, were found to display molt cycle-related differential expression profiles.
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