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A 3' and/or 5' stem-loop structure may be designed to increase specific binding to difficult sequences.
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FOXP3-ΔN binding to ACAAACA sequences was further decreased, and binding was altogether abolished to GCAAACA sequences (Figure 3B).
Candidate Zinc-finger binding to repetitive sequences.
By binding to complementary sequences (a.k.a.a
CDX2 regulate MUC2 [ 16] by binding to enhancer sequences [ 17].
TFs are families of small proteins that regulate gene expression by binding to specific promoter sequences.
Gfi1 is a transcriptional repressor binding to cognate binding sequences in regulatory elements [38], [39].
Protein binding to endogenous and mutated sequences was therefore investigated.
The development of the compounds preferentially binding to the specific DNA sequence is one of the potential but very difficult approaches in this strategy.
Finally, obtaining structural information of weak inhibitors binding to ion channels is notoriously difficult.
Discovery of a binding site for WT1 has proven difficult since each isoform of the regulator may bind to slightly different sequences in DNA.
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