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Some fluorescent probes have specific binding to different regions of the HSA, and the binding sites can be determined by the displacement binding experiments using some probes.
These compounds bound to the pocket with an affinity similar to locostatin; however, their side chains allowed for binding to different regions within the RKIP binding pocket.
Together, these observations are consistent with M2- and M3-APC binding to different regions of topo IIα and thus modifying topo IIα activity by slightly different mechanisms.
For the detection of mAbs binding to different regions of COMP molecule, we performed ELISA with the recombinant COMP fragments.
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These ligands may occupy very similar and overlapping binding regions, while the rest of the ligands bind to different regions within the QZ59-RRR-associated binding site.
We further investigated [ 18 F]flutemetamol binding to different brain regions and structures inside the head in WT male C57Bl/6N mice (N = 4) using ex vivo tissue counting to better understand the observed binding in vivo.
Primer for prfA-analysis are lmo0200F3 (5′-GTATCACAAAGCTCACGAGT-3′) and lmo0200R3 (5′- TGTATCAATAAAGCCAGACAT-3′). Primer binding to different genetic regions for microarray confirmation are listed in Additional file 3. 16S rRNA was used to normalize the qRT-PCR data as described previously [ 92].
Further experiments should investigate direct AR binding to different PCA3 genomic regions, in order to answer these open questions.
In fact, different ligands of PXR can modulate PXR binding to different response elements in the promoter regions of targeted genes [ 20].
Therefore, a set of fusions of OspA to different regions of factor H binding protein (fHbp), a meningococcal surface-exposed lipoprotein, were designed and tested for their surface-exposure.
These unstructured regions can adopt different conformations to facilitate binding to different host target proteins.
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