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A review study summarized the types of protein binding to different nanoparticles to determine the composition of protein corona [40].
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Some fluorescent probes have specific binding to different regions of the HSA, and the binding sites can be determined by the displacement binding experiments using some probes.
This stabilizing effect seems to apply to chemically different nanoparticles.
Thus diarylethene derivatives with TSC and MTSC side-chain groups despite their similar structure have different optical response and interact variously while binding to gold nanoparticles.
Second, a smaller amount of aptamers can be used for binding to gold nanoparticles, in contrast to thiolated aptamers where significant amounts of aptamers are necessary to coat and stabilize the gold nanoparticles.
Thio-PEG has a very high affinity to gold nanoparticles, which makes it possible for thio-PEG to block antibodies from binding to immunized gold nanoparticles during the synthesis process.
Tf binding to the nanoparticle surface did not significantly affect the size characteristics of the vesicles.
When nanoparticles enter a biological fluid, proteins rapidly compete for binding to the nanoparticle surface, leading to the formation of a protein corona that critically defines the biological identity of the particle.
To examine nanoparticle binding to TCR, we synthesized nanoparticles bearing MHC-Ig alone, thus removing the binding contribution of anti-CD28.
The QDs, due to the ease of multiplexed detection, serve as a robust targeting model for various nanoparticles, demonstrating that this approach can specifically target three or more different nanoparticles to genetically specified locations, as long as those nanoparticles are available as biotin-binding conjugates.
These findings emphasize the existence of different mechanisms of cytotoxicity of different nanoparticles and point to protective strategies to overcome nanoparticle-induced adverse effects at the cellular level.
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