In support of the possibility that ZNF274 may use different sets of fingers to bind to different motifs, we identified several different motifs that were highly enriched in the ZNF274 binding sites.
The affinity of Xbra binding to different DNA motifs was measured on an Octet RED biolayer interferometer.
For example, our data indicate that CREB1 preferentially facilitates GABPα-GST binding to different SNPs within the two ETS motif variants (C/CGGAAGT and /GCGGAAGT), highlighting the detail in these data.
In rare cases the same motifs bind both classes of importins, for example the adenovirus core protein pVII [46], while in other cases, like the guanine nucleotide binding protein GNL3L [47], separate motifs bind to different importins.
Particularly, CaM binds to different types of target binding motifs, where the hydrophobic residues are separated by 1 10, 1 14 and 1 16 residues [11].
Each HLA allotype binds to different ligands characterized by specific binding motifs [ 91].
Therefore, we can not exclude the possibility that the paralogous copies of S4 in Clostridia are regulated by Zur binding to a motif different from any in our profile.
The 30 kDa bilobal C-terminal appendages of α and β2 each possess two sites for binding different motifs found on many other endocytic CCV coat proteins with various accessory/regulatory functions in CCV formation (reviewed in Owen et al., 2004; Schmid and McMahon, 2007).
At the onset of differentiation, p38 is activated and phosphorylates E47 at Ser140, leading to its dimerization with MyoD1 and to subsequent binding to E-box motifs of different muscle promoters.
Since we have demonstrated that the percentage of TLR9 predimers did not increase upon addition of CpG or non CpG DNA, the binding affinity of TLR9 to different DNA motifs might play an important role in its activation.
