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mAb 215F12A4 (IgM) in vitro selectively recognized Tn on the MCF7 surface, since its binding to cell membrane was completely abolished by preincubation with purified Tn.
The modification in these region leads to an irreversible lost of enzymatic activity of sPLA2 and/or its ability for binding to cell membrane [ 27].
New information on the peptide binding to cell membrane properties, which are the grounds of intracellular signalling, is a fundamental contribution to this cytotoxic peptide research field [ 28, 29].
54, 55 Myofibroblasts express integrins that can bind to the LAP, and mechanical stress applied to the integrins, either by mechanical stress on the matrix and/or via myofibroblast contraction, can effectively activate TGF-β1 without cleaving the LAP and allow its binding to cell membrane receptors.
Binding to cell membrane, followed by translocation into the cytosol and RNA degradation, is a necessary requirement to convert a ribonuclease into a cytotoxin for malignant tumor cells.
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Similarly, the presence of cholesterol in incubation medium may bind to LLO, which in turn may prevent its binding to cell membranes.
In the process of our experiments, we found that NPs binding to cell membranes occur within few minutes under magnetic field.
These results show the potential of asymmetric liposomes for the targeted binding to cell membranes in response to (external) temperature stimulus.
After knocking down the expression of EGFR in HSC-T6 cells using an EGFR-shRNA expression plasmid, immunofluorescence labeling showed that MD-1 binding to cell membranes decreased accordingly (Fig. 4B).
An alternative mechanism might involve the binding of poly-APS to cDNA, this complex binding to cell membranes and then internalisation by endocytosis.
Inconsistent with the results in Figure 1E, cholesterol addition did not prevent cell apoptosis but even slightly promoted cell apoptosis, which was further verified by measuring Annexin V binding to cell membranes using flow cytometer analysis.
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