Sentence examples for binding to an isolated from inspiring English sources
Assuming that binding to an isolated site reflects direct TM actin contacts and cooperativity coefficient reflects long-range interactions induced by the TM that is already bound, our results suggest the C-terminal region can affect the binding of tropomyosins through both direct contacts and/or long-range interactions.
The decrease of the actin affinity is due to either reduced binding to an isolated site, with no adjacent binding of TM molecules, or it is due to reduced binding cooperativity.
Further, the apparent binding affinity determined with electrophysiology reflects cooperative ligand-binding to the full length tetrameric channel whereas the binding affinity determined with tryptophan fluorescence reflects ligand binding to a monomeric, isolated C-terminal domain and therefore lacks effects of cooperative interactions.
However, it was surprising that we did not see a loss of Dsk2 binding to proteasomes isolated from an rpn13Δrpn1-VKD strain.
Competitive ligand-binding assays measure the binding affinity of a substance to an (isolated) receptor.
The focus was on tests based on receptor binding and receptor activation in cell cultures measured via reporter gene activation or cell proliferation: Competitive ligand-binding assays measure the binding affinity of a substance to an (isolated) receptor.
The analysis revealed that in the case of three isoforms (smTM, TM1b9a and TM5a) the affinity to an isolated binding site (Ko) was reduced and binding to a contiguous site was increased by modification of the actin C-terminus.
Adapting the McGhee and von Hippel model for ligand binding to a linear lattice [33], we analyzed the effect of actin truncation on TM affinity to an isolated binding site and an equilibrium constant for moving TM from an isolated binding site to a singly contiguous binding site, which is a measure of binding cooperativity [11,31,34].
Finally, we assessed whether fucosylation plays a role in CTB binding to freshly isolated colonic epithelium.
One HCD peptide, B38, showed high affinity binding to both isolated HDL and LDL, and could exchange between lipoproteins.
