CsrA has a clearly defined specificity based on binding to a specific mRNA sequence to inhibit translation.
However, Dr. Jensen used radioactive tracers to show that steroid hormones actually affect cells by binding to a specific receptor protein inside them.
Many proteins can switch from one conformation to another under the influence of an external driving force, such as the binding to a specific substrate.
With the advancement in information technology, the dynamic understanding of drug binding to a specific target protein can be achieved using molecular dynamics simulation (MD) approach.
At the heart of the screening for fragments binding to a specific target, crystallography delivers structural information essential for subsequent drug design.
The selectivity of strong binding to a specific sequence can allow for quadruplex sensing and the detection method presented here is very simple, using fluorescence and NMR study.
How proteins achieve high-affinity binding to a specific protein partner while simultaneously excluding all others is a major biological problem that has important implications for protein design.
MET4 targets overexpressed c-MET in tumors by binding to a specific epitope located in the extracellular domain of c-MET.
Micro-RNAs silence gene expression by improper binding to a specific or to multiple mRNA sequences into a ribonucleoprotein called RISC (RNA-associated silencing complex).
This method gives information on the amount of ligand binding to a specific complex with a thermodynamic constant, Ktherm, that differs from Kcond ML.
Again this is highly relevant for screening where the biological end point is based on phenotypic changes rather than on binding to a specific target.
