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The resulting eightfold mutant, CaM_8, was constructed and tested for binding to a set of seven CaM target peptides.
The spectrophore is a novel descriptor that reflects, in a virtual manner, the way how molecules are binding to a set of artificial receptors, taking into account the spatial interactions between a molecule and its surroundings.
The extracellular actions of S1P are mediated by binding to a set of S1P GPCRs (G-protein-coupled receptors) (S1PR1 S1PR5 or S1P1R S1P5R) [ 4].
Although TFs in the same cluster can be involved in remote events in different cell types in the same organ or even whole plants, depending on how the samples are harvested, the confirmation of subset TFs binding to a set of target genes is still helpful in consolidating the cluster.
Assessment of p53 binding to a set of 14 activated and 4 repressed novel p53 targets by ChIP-PCR, as well as their differential expression using quantitative PCR (qPCR), both in MCF7 and HCT116 cells, confirmed that the selected genes are novel p53-target genes.
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After duplication of the ancestral SR, three mutations in one copy radically weakened binding to the ancestral estrogen response element (ERE) and improved binding to a new set of DNA sequences (steroid response elements, SREs).
In a dot blot assay, individual yBRDs binding to a complete set of the histone peptides spotted on the membrane were evaluated with Western blotting using anti-GST antibody (Figure 2A).
In their active GTP-loaded state, Ras G-proteins can transmit signals by binding to a specific set of proteins termed effectors.
As a measure of self-reactivity, we tested IgM Abs for binding to a defined set of Ags, including ssDNA, dsDNA, and histone, by ELISA in culture supernatants from 10 healthy donors.
The family of RTKs for example, though expressed and employed in a functionally diverse manner, all transmit signals across the plasma membrane through binding to a limited set of adaptor proteins.
A range of binding profiles is observed, from very restricted binding to a small set of structurally similar ligands to binding of broad classes of ligands with related terminal sugars and even to failure to bind any of the glycans on an array.
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