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Among the cytokines produced by different tissues, VEGF (Vascular Endothelial Growth Factor) mediates its biological effects by binding to a series of transmembrane tyrosine kinase receptors, principally VEGFR-1 and VEGFR-2 [9].
For this, we used the HaloTag protein (Promega Corp ., which is a genetically modified hydrolase that is able to catalyze its covalent binding to a series of membrane-spanning ligands and can be used for in vivo labeling [15].
RAD51 interacts with BRCA2 by binding to a series of BRC repeats that are present in BRCA2 [ 21, 32].
Antigenicity studies of recombinant DI produced by E. coli were expanded to test binding to a series of affinity purified polyclonal IgG samples derived from patients with APS.
To investigate the approximate footprint of DNA recognised by Chd1-DBD, binding to a series of progressively shorter DNA fragments was tested.
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In order to study electron transfer trends between metal complexes binding to DNA, a series of complexes [M phen 3]n+ [M=Os II), Ru II), Co III) and Zn II); phen=1,10−phenanthroline, n=2 or 3] were computed using the DFT-B3LYP method and LanL2DZ basis set.
To determine the minimal requirements for specific HCV core peptide binding to DDX3X, a series of N-terminal GST fusions to HCV core peptides were used for pull-down assays (Figure 1B).
To determine whether the NPxY motif is required for Pafah1b2 and Pafah1b3 binding to VLDLR, a series of C terminal truncation mutants of the receptor tagged with GFP were produced (Fig. 2A).
These data reveal that after downstream P140 binding to HSC70, a series of molecular events take place affecting the APC functions of MRL/lpr B cells, leading finally to a decreased expression or stability of MHCII molecules and a potential reduction of autoreactive T-cell signalling and priming.
To determine which region of ASB9 is essential for binding to uMtCK, a series of Myc-tagged ASB9 deletion constructs that encode various numbers of ANK repeat motifs was made and cotransfected with haemagglutinin (HA -tagged uMtCK constructs into HA -taggedls.
Although the hydrophobic stripe on TRIAP1 seems somewhat tolerant to mutation, this tallies with its role as a chaperone, in that the overall shape and hydrophobicity of the interactional surface are important for its ability to binding a series of related but not identical PRELI-like substrates.
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