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For all regression analyses involving islet and UK blood samples, expression data were generated using the CDKAL1-v1 assay without an oligonucleotide binding to a sequence overlapping rs9366357.
Takagaki and Manley used a recombinant form of CstF-64 that included only the RNA recognition motif (RRM) and found preferred binding to a sequence that included both GU-rich (G(U 2 4G) and U-rich ((GU 2 4) components [ 17].
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Additionally, if a transcription factor shows degeneracy in its TFBS [ 44, 45], binding to a CpG sequence and a similar sequence without a CpG, it suggests that this transcription factor is involved in both constitutive and regulated gene expression.
Furthermore, H. pylori Fur autoregulates its own synthesis – inducing synthesis when iron is limiting by binding to one regulatory DNA sequence, and repressing synthesis when iron is abundant by binding to a nearby sequence (Fig. 1) [4]–[6].
Evaluate a C2H2 zinc finger transcription factor for binding to a DNA sequence.
This transcription factor acts by binding to a consensus sequence called catabolite-responsive element (cre), usually found in the promoter region or the 5′ part of catabolite regulated genes18.
miRNAs work as post-transcriptional regulators by binding to a target sequence located within the 3′ UTR of the mRNA, promoting degradation of the mRNA or inhibiting translation of the mRNA.
The selectivity of strong binding to a specific sequence can allow for quadruplex sensing and the detection method presented here is very simple, using fluorescence and NMR study.
Antisense oligonucleotides act as exogenous inhibitors of gene expression by binding to a complementary sequence on the target mRNA, preventing translation into protein.
The bNAbs against MPER binding to a peptide sequence obey the Langmuir curve model, but SPR-based studies demonstrated that binding polypeptide-membrane complex follows a two-step (encounter docking) model.
Instead, Morpholinos act by "steric blocking", binding to a target sequence within an RNA and simply getting in the way of molecules that might otherwise interact with the RNA.
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