Probes showing strong secondary structures or binding to a region of the target with such a strong secondary structure were not used.
The identification of different ligand binding modes to a secondary site can provide hints for the design of non-ATP-competitive inhibitors targeting the interaction between the α catalytic and the β regulatory subunits.
After binding to indicated secondary antibodies, an enhanced chemiluminescence (ECL) blotting analysis system (GE Healthcare Life Sciences, Piscataway, NJ) was used for antigen-antibody detection.
Observed rates of hydrolysis were fit to eq 1, which describes the activation of the enzyme (when b > 1) at relatively high BTC concentrations from the binding of the substrate to a secondary binding site 1that afforded best-fit kinetic values and relevant standard errors.
The reason for the stimulation could be an allosteric effect by glucose binding to the secondary site [ 13, 34] or the occurrence of transglycosylation [ 32].
The weakness of all these models is that they presuppose secondary B cells binding to and processing secondary antigens in the absence of T cell help.
Recent results [ 15]suggest that the interactions of chlorpyrifos oxon with AChE are complex and may involve the binding of this oxon to a secondary site on the enzyme.
These cadherins facilitated toxin monomer oligomerization and mediated oligomer binding to secondary receptors.
The predominant assumption is that highly selective ligands would avoid unwanted side effects caused by binding to secondary non-therapeutic targets.
Finally, to restart replication following ICL processing, FANCJ might similarly prevent MSH2 binding to secondary structures that form at arrested forks that are barriers to replication.
