A tacit assumption of 3-D methodologies is that chemical structures with similar shape and binding features will have similar (if not the same) mode of action of "activity", e.g., of binding to a protein binding pocket in the same fashion.
In contrast, we found less than 0.6% non-specific binding to a control NP366 374/H-2Db dimer by the same EAE splenic single-cell suspensions (Fig. 1a).
Here, the IDR acts as a competitive inhibitor of other regions in the same protein for binding to a partner.
The second main phase is the fragment-based screening[ 3b of the biofragment library for binding to an uncharacterized protein from the same or similar family from which the biofragments were derived.
Interestingly however, caveolin-3 binding to this same site via a WW-like domain [ 36] appeared insensitive to the tyrosine phosphorylation [ 28].
It has also been widely suggested that microRNAs, similarly to transcription factors, can act in combination (or cooperatively) by binding to the same mRNA in a concentration-dependent manner [ 41].
Because the property of being constitutive requires binding to the same locus in a variety of cell types, the number and diversity (or lineage) of cell lines/types providing data to the algorithm would be expected to have a strong influence on the biological trustworthiness of any result.
A series of molecules binding to the same protein active site is drawn in a graphically consistent way.
Unlike CO, where a partial positive charge exists on carbon when bound to a metal ion, cyanide is not subject to attack by hydroxide and functions as an inhibitor, binding to the same site as CO without initiating catalysis.
Binding to the lectins was specifically inhibited by a monosaccharide or a disaccharide that competes the binding to the same lectin, which confirmed the binding signal was specific.
