Antibody binding to a high-entropy molecule would require a greater activation energy than binding to a lower-entropy molecule and may thereby introduce structural or functional constraints in either the FWR or CDRs.
Scatchard analysis shows binding to a single class of lower affinity sites (Kd - 0.7 +/- 0.15 nM).
Mutation of the GATA sequence shifted the DNA binding complex to a lower molecular weight, consistent with the absence of GATA-4 (Fig. 4B, lane 4 5 from the left).
Mutation of Lys-1370 to Ala resulted in a 4-5-fold increase in the Kd for binding to the lower affinity site with no significant alteration in binding to the high affinity site or signal transduction properties.
Direct binding studies with K1374R demonstrated no significant alteration in binding to the lower affinity site; however, binding to the high affinity site is reduced by 83%.
Receptor-associated protein competes with RBF for binding to the lower but not the higher affinity site.
Two hours of pretreatment with 2.5 and 10 mmol/l glucosamine significantly reduced c-jun DNA binding activity (P < 0.03; Figure 7), whereas junD binding activity was reduced to a lower degree (data not shown).
From a law of mass action perspective, keeping gene-specific transcription factor abundance low, i.e., spanning the Kd values of the best binding sites, could prevent them from binding to lower affinity sites, with an undesired transcriptional effect [ 46].
Although this indicates that residues of the 41 45 region (particularly His and Ile) play a key role in the binding of cyclin Y to PCTAIRE-1 that involves 14-3-3 14-3-3 14-3-3annot rule out the possibindingthat truncation or mutation (i.e. alanine substitution) may have disrupted normal protein folding of cyclin Y, although 14-3-3 binding is retained (although to a lower extent).
Figure 6a right panel illustrates that in spite of the high abundance of low molecular mass components, only dimers and HMM aggregates of ∼150 350 KDa (as estimated by Fergusson plot analysis) displayed specific binding to DR4 and, to a lower extent, DR5, in agreement with the immunofluorescence data.
Examined strains had a hemagglutinin polymorphism associated with enhanced binding to lower respiratory tract receptors.
