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It has been previously documented that low lipid or detergent concentrations promote fibrilization of αS (1− 4, 6, 58), citing as cause the increased local concentration of αS when many molecules compete for binding to a limited surface area.
The family of RTKs for example, though expressed and employed in a functionally diverse manner, all transmit signals across the plasma membrane through binding to a limited set of adaptor proteins.
Under these conditions, Smc1 and Smc1-myc9 compete for binding to a limited amount of Smc3-HA3, wasch was immunoprecipitated from cell extracts and proteins analysed by SDS PAGE and silver staining.
By the different permutations of co-regulators, even a small number of DBTFs binding to a limited number of cis regulatory elements can form a system, which can execute the innumerable gene expression profiles that are necessary for sustaining eukaryotic organisms.
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The most readily interpreted results on the glycan array are those that reflect binding to a very limited set of glycans that clearly share a structural motif.
The mutant or wild-type specific PCR amplifications take place in the same reaction tube, thereby allowing the mutant or wild-type specific PCR primers to compete for binding to very limited templates.
In this context, rational approaches using nucleotide analogues to target RecA's ATP binding site have been examined to a limited extent.
Such saturable binding of [F]Lymphoseek 3 to a limited number of SLN mannose receptors (32, 34, 41) suggests that conjugation to 1 does not alter Lymphoseek's mannose receptor specificity.
In a different situation, transient binding to standalone proteins with a limited choice of affinities is able to induce this kind of slowdown [38], [39].
The single domain APOBEC3A protein shows no RNA binding ability except a limited amount to doubled stranded RNA, despite sharing the preferential targeting to promoters as the rest of the deaminases.
Horseradish peroxidase was used as an enzyme-labelled antigen in both assays that made a competition with unlabelled antigen for binding with a limited number of antibody sites on the micro-plates (solid phase).
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