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The HIV envelope protein (Env) is essential for binding to a human lymphocyte or T cell.
Protein variants evolved from the scaffold of CBM4-2, from the xylanase 10A of Rhodothermus marinus, were selected in a previous study [ 28] for binding to birch wood xylan or binding to a human IgG4 molecule.
In the three cases examined, PEG linkage improved binding affinities of ADP3 greatly against chicken IgY, improved NMOP6 binding to a human IgG1 protein modestly, and had little effect on binding of FLAG peptide to anti-FLAG antibody.
A similar case was reported for TMPyP4 binding to a human c- kit proto-oncogene DNA G-quadruplex with a strong binding constant (K1) around 10, and a weak K2 around 10 [ 41].
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To ensure that the CPE-containing fusion toxins retained receptor binding, their ability to bind to a human ovarian carcinoma cell line was assessed using an ELISA-based binding assay.
Here, we demonstrate a high-throughput methodology allowing rapid identification of the proteins binding to a given human RNA molecule using a protein microarray containing ~9,400 human recombinant proteins spotted in duplicate.
To corroborate whether PAB binders are capable of binding to a native human target, we used LNCaP prostate cancer cell line formerly described to secrete and cell-surface express the PSP94 protein (Yang et al., 1998).
Background binding to an irrelevant human IgG, MonoD, was subtracted.
A functionally-validated in silico-in vitro approach to the reliable and efficient prediction of peptide binding to a previously uncharacterized human MHC allele HLA-Cw*0102 HLA-Cw*0102ed.
Mutations identified in people with dyskeratosis congenita can prevent the DKC1 complex from binding to a subset of human RNA molecules.
In this context, we have been unable to reproduce the reported results supporting the notion that recombinant visfatin may act as an extracellular pro-inflammatory cytokine through binding to a putative receptor on human PBMCs, even at doses above the physiologocal levels (results not shown).
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