The main peak of SUNBRIGHT® ME-050MA conjugated NFG1CG4-hFasLECD was eluted under significantly lower NaCl concentration (6.3 mM), suggesting weaker binding to a negatively charged column carrier due to the reduced ionic interactions caused by the possible shielding effect of the attached PEG moieties.
However, NPA functions as a general inhibitor of secretory processes (by binding to a putative NPA-binding protein), associated with the auxin efflux carrier site [ 31, 35].
Subsequently, ACTH increases cortisol release from the adrenal glands, which then binds to a specific carrier – cortisol-binding globulin (CBG) – that is synthetized by the liver and to albumin in order to reach the target tissues.
It typically involves binding the enzyme to a carrier (support), such as an ion exchange resin or silica, or encapsulation in an inert carrier, affording a biocatalyst with superior operational performance compared to the free enzyme.
To rule out that the chaperones were binding to the carrier GST, AtClpC2 and AtClpD were added to a GST-containing resin.
In embryonic neurogenesis, the homeodomain transcription factor PROS is expressed in the NB but it does not enter the nucleus due to its binding to the carrier protein MIRA, which localizes to the cell cortex.
In the present study the boronated derivative 5 demonstrated preferential efficacy in PDT of melanoma B16 and sarcoma M-1 compared with its boron-free counterpart 2. One reason for this effect might be an elevated binding to albumin, a major carrier of many biomolecules [24].
Use of a short chain rather than full length peptide improves the potential for conformational binding of active peptide to a carrier surface.
Use of a short chain peptide rather than full length protein is cost-effective and improves conformational binding of active peptide to a carrier surface.
This can be achieved by binding the steroid hormone to a carrier macromolecule that can be a large protein.
