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We conclude that Hox proteins exhibit clear target specificity when binding to Kc167 cell chromatin.
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Fig. 2 Displacement curves of [177Lu]CP04 binding to CCK2+ cell membranes using increasing concentrations of AAZTA-MG, [natGa]AAZTA-MG, [natLu]AAZTA-MG and CP04 as control.
Shikonin also did not inhibit monocyte chemoattractant protein-1 (MCP-1) binding to CCR2 cells, eotaxin binding to CCR3 cells, interferon-inducible T cell α-chemoattractant (I-TAC) binding to CXCR3 cells and SDF-1α binding to CXCR4 cells.
Negligible [3H]DAMGO binding to A7 cells suggested that these cells (and presumably their M2 parent line) do not express MORs (Fig. 2B).
All affinity matured variants showed an increased binding to T47D cells, which are the tumour cells used for the initial selection of IIB6.
Here, all IgG varants bound weaker than their scFv-Fc analog with HT186-D11 showing the best binding to T47D cells, and huHMFG1 (only analysed as IgG) showing the lowest binding to MUC1.
Inhibition of fucosylation reduced CTB binding to Colo205 cells, too.
To measure CTB binding to Colo205 cells, we used flow cytometry.
Gangliosides do not make a large contribution to CTB binding to Colo205 cells.
NB-DGJ treatment did not affect CTB binding to Colo205 cells.
We found that Neu5Ac strongly inhibited CTB binding to T84 cells (new data in Figure 6C,D), but only slightly inhibited CTB binding to Colo205 cells (new data in Figure 6F,G).
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