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The anion binding titrations indicate that weak 1 1 binding is dominant with additional contributions from 1 2 gelator/anion complexes, consistent with the widely separated disposition of the two urea functionalities.
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Results from EMSA experiments and NMR titrations indicate that the binding affinity of JAZ zinc fingers for RNA is dependent on the length of the A-form helix.
The titrations indicated that cerium and aluminium compounds precipitate independently.
Data analysis of Ca2+ binding affinities to NCaBD obtained by ITC titration indicates that the Ca2+ binding to EF1 and EF2 could be explained by a model with two independent binding sites or identical intrinsic binding with a slightly negative cooperative.
The fluorescence titration indicates that the mutated EF2 still retains its Ca2+-binding ability, albeit much weaker, which is affected by the Ca2+-bound EF1 in a positive cooperative manner.
1H NMR titration indicates that the mechanism of sensing involves formation of H-bonding between fluoride ion and imidazole NH and phenolic OH moieties simultaneously with fairly high binding constants (105 M−105
Although peptide array experiments were suggestive of an interaction with S100B the titration indicates that the affinity is very weak.
In contrast, binding studies using isothermal titration calorimetry indicate that Diva does not bind peptides comprising the BH3 region of several proapoptotic Bcl-2 proteins, including Bak and Harakiri [22].
ITC and quantitative NMR titration experiments indicate that JAZ ZF1, ZF2, and ZF3 have similar binding affinities for VAImin RNA and that the 2 1 (ZF/RNA) stoichiometry of the complex is conserved.
Moreover, fluorescent titration experiments indicate that the two copolymers have high sensitivity for Hg2+.
Titration runs indicate that normalization is not normally necessary when thousands of sequences from a single tissue type are considered.
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CEO of Professional Science Editing for Scientists @ prosciediting.com