Sentence examples for binding tissues were incubated from inspiring English sources

To control for specific binding, tissues were incubated with TUNEL reaction mixture that lacked labelled nucleotides.

To block nonspecific binding, tissues were incubated with 1% BSA or 2% goat serum in PBS for 30 min.

To control for specific binding, tissues were incubated with irrelevant rabbit IgG antibodies and appropriate fluorescent secondary antibodies, or were incubated with secondary antibodies alone.

To block non-specific antibody binding, tissues were incubated in 10% fetal bovine serum/Tris-buffered saline with Tween 20 for 30 minutes.

As a control, cells or tissues were incubated with secondary antibodies only and no non-specific binding was observed.

After blocking endogenous peroxidase activity with 3% hydrogen peroxide and 97% methanol, the tissues were incubated in 3% bovine serum albumin (BSA) for 1 h to eliminate non-specific binding.

Nonspecific binding was blocked by incubation in 5% goat serum in phosphate-buffered saline (PBS) for 15 min, and the tissues were incubated with polyclonal rabbit anti-SASH1 antibody (1 : 100, Santa Cruz Biotechnology Inc., CA, USA) overnight.

For thrombogenicity and immunogenicity testing tissues were incubated with human blood.

Tissues were incubated in 0.125% collagenase (Sigma, C0130) solution to achieve single cell suspensions (15 min, 37 °C).

After several washes, all tissues were incubated with DAPI (1 200 in PBST) at room temperature for 20 min.

The sections from paraffin embedded tissues were incubated with anti-S100 β (1 200, Millipore, MA, USA) at 4 °C overnight.