Sentence examples for binding the samples were from inspiring English sources

After binding, the samples were washed with lysis buffer for three times.

After binding, the samples were washed with lysis buffer three times.

To reduce nonspecific antibody binding, the samples were exposed to 2%% bovine serum albumin (BSA) for 30 min.

To eliminate nonspecific protein binding, the samples were incubated with 10% normal goat serum for 30 min at room temperature.

After incubation for 30 minutes at 4°C for binding, the samples were washed 5 times with washing buffer (50 mM Tris-CL, 75 mM NaCL, 10 mM MgCL2, 1 mM DTT, 0.01% Triton-X100 and complete EDTA-free protease inhibitors; Roche).

To assess the reliability of differential AR binding events, the samples were shuffled randomly based on 56 available permutations.

For repetitive photomodulated binding cycles the samples were reincubated under visible white light until equilibrium was reached.

After adding additional 400  μl of 1 × binding buffer, the samples were analyzed by flow cytometry within 1 h.

The binding energies for the samples were calibrated by setting the measured binding energy of C 1s to 284.60 eV.

To prevent non-specific binding of the antibodies, the samples were blocked in 10% goat serum (Sigma) in PBSTX for 1 hour at room temperature.

To block nonspecific binding of the antibodies, the samples were incubated with 1 % bovine serum albumin (BSA) in PBST for 1 h and washed twice with PBST, with each wash lasting 5 min.