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An avidity assay was developed to measure the strength of DENV antigen antibody binding; the procedure was similar to assays previously described.
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The procedure was postponed.
The procedure is straightforward.
The procedure is classic.
A competitive binding assay procedure was performed: A 96-well plate (MICROLON plaque 200, med. Binding, F-bottom, GREINER) was coated with 200 μL/well of phosphatidylserine (PS) (Avanti Polar Lipids) (dissolved in ethanol (SIGMA ALDRICH) for a final concentration of 100 μg/mL).
Using 32 MHC peptide binding predictors, the same procedure was followed to plot the average density of MHC-binding peptides, and the average density of CTL epitopes.
The binding assay procedure is the same as described above.
The procedures were clear.
For RNA binding assay, this procedure was repeated with using total RNA of HeLa cells instead of hyaluronan.
The difference between DRO and FMIPv6 is that, under DRO, the binding update procedure is performed in a forward manner such that the MR implements the handoff procedure concurrently in the network and link layers.
In order to assess nonspecific binding of the secondary antibody, the same procedure was carried out in the absence of primary antibody.
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CEO of Professional Science Editing for Scientists @ prosciediting.com