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Ligand interaction was however inhibited by steric hindrance associated with the bulky phenyl ring system thus affecting the increased binding that could be realized from the improved hydrophobic nature of the molecules.
The slow migration of LipC seemed to arise from the lack of SDS binding that could be deterred by the unusually high negative charge amino acids occupying about 17% of the total MW.
CR56 also gave saturable binding that could be fitted to two tight binding sites (Table 3).
However, in vivo evaluation of [I] 10 failed to demonstrate any binding that could be attributed to VGSC expression.
Although Zuurbier and colleagues have challenged these conclusions (Nederlof et al., 2013), we sought other means to modulate mitochondrial HK2 binding that could be used to investigate its role in cardioprotection (Pasdois et al., 2012).
Larsen and colleagues postulated that the hyperdynamic circulation in ALF is attributable to a circulating vasodilating substance or "humoral factor", perhaps endotoxin, with a high molecular weight, or high degree of protein binding, that could be cleared using the filter used for TPE [ 10, 11].
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Further structural characterization of eIF4E complexes revealed unique binding elements in different eIF4E binding partners that could be fused into a chimeric '4E-BP-like' mimic and dampen translational activity [ 98].
It is possible that fusing GFP to the amino terminus of Cx43 may be blocking Cx43 binding proteins that could be required for efficient tumor cell diapedesis, although, to date, no amino-terminal Cx43 binding proteins have been identified.
Because all of these structures have more than one ligand that binds to protein, we had a large number of ligand-protein binding structures that could be used for in silico drug screening using chooseLD.
Examples of five estrogen receptor binding compounds that could be entered in a profile query.
In case of the mixture with gum arabic, a decrease is observed in the fluorescence attributed to the binding medium that could be explained by a quenching effect [41].
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