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They performed binding tests for streptavidin after attachment, and found that biotin was still able to bind streptavidin.
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Bacterial strains showing positive virulence by Congo red binding test were chosen for the studies.
The HS guidelines revised in 2011 [ 16] show that the EMA binding test is useful for screening atypical HS patients.
Potential SP1 binding was tested for 4 candidate target genes containing one or two best 20% SP1 and TFAP2A binding sites by Chromatin Immuno Precipitation (ChIP) assay, as indicated by Jaspar PWMs.
The unlabeled binding partners tested for KD determination were KA (0 4 mM), HQ (0 1 mM) and L-tyrosine (0 2 mM).
The effect of modification on the binding was tested for three different types of DNA junction.
(E ) Y2h assay in which CHMP1B WT and deletion and point mutants fused to Gal4 DNA-binding domains were tested for binding to ULK3 fused to the VP16 activation domain.
A 79-nucleotide-long promoter fragment, which contains four W-box or W-box like elements, was indicated, and used for DNA-binding test in yeast.
Tests for promiscuous binding were done using Blastn.
The dominant criticism of HTPS applies to QSAR as well – it tests for receptor binding only.
SVOP-E450A, SVOP-Y425A, SVOP-R457A and the double mutant, G398A/R399A, which is analogous to residues required for nucleotide binding to Glut 1 [30], were tested for binding to 8-azido ATP.
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