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Eosin-5′-maleimide (EMA) binding test is a flow cytometric test used to detect hereditary spherocytosis (HS).
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The EMA binding test is believed to be a specific method for diagnosing HS.
EMA binding test is used as a screening test to diagnose HS [ 3, 7– 16].
However, an EMA binding test is commonly performed on neonates and infants younger than 6 months, as a first-line test in HS screening.
The HS guidelines revised in 2011 [ 16] show that the EMA binding test is useful for screening atypical HS patients.
The competitive binding test was performed by using gallic acid along with syringic acid.
Bacterial strains showing positive virulence by Congo red binding test were chosen for the studies.
Compressive strength of LV5 is elevated with disruptions of Sost and Sfrp4, whereas the strength of the femur shaft determined by three-point or four-point binding tests is greatly increased in bones from Sost KO mice.
To validate the assay conditions, a resin-binding test was performed.
A nonspecific protein-binding test was also carried out to show that the PEG coating can help reduce the protein adsorption onto the surface of the particles, relating to the biocompatibility of these PEG-coated particles.
The results of in vitro binding specificity test are presented in Fig. 3.
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