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Only 19 (5.5%) and 7 (2.9%) of the 903 putative direct binding targets were found in the up- and down-regulated genes of GS1.
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In the E83 group 80 of the 83 input sequences were found to have a NF κB binding sequence, with 21 of the 22 known targets being found.
Using this pull-down assay, many potential RNA targets for puf-A bindings were found and listed in Table 2 with their gene IDs and symbols.
Replacement of 19 amino acids in Streptomyces griseus trypsin targeting the active site and the Na+-binding site were found necessary to generate efficient Na+ activation.
STAT3 target genes were found to be downregulated and a decrease in STAT3 DNA binding was observed following LLL12 treatment, indicating that LLL12 is an effective STAT3 inhibitor.
513 additional drug-target interactions were found.
After integrating operon structure information, 2445 regulator-target gene relationships were found to comprise the final regulator-target gene binding site map.
Because previously described mAbs targeting the C4 region were found to block CD4 binding of gp120, we determined the binding specificity of R53 using a competition ELISA.
When comparing gene expression signatures of EWS/FLI1 with the published ChIP-chip and ChIP-seq results, less than 10% of EWS/FLI1-regulated genes were found to be binding targets by these methods [ 23], suggesting that either the vast majority of EWS/FLI1-responsive genes are indirectly regulated or a large number of direct EWS/FLI1 targets were missed.
The ternary complexes having a targeting-ligand, transferrin (TfP), were found to be especially effective at binding to the TfP receptor rich cancer cells, HCT119.
This study assumes that expression levels of the TFs explain less target gene expression if their binding locations are found further away from the target gene's TSS.
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