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Second, the three knockout genes are transcription factors, and their binding targets can be obtained from ChIP-chip data.
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Upon functionalization to enable specific binding, magnetic particles and their targets can be conveniently positioned in vitro and in vivo by the distal application of magnetic fields.
Furthermore, it has to be noted that even for those indicators where binding political targets exist, these targets can be revised accordingly if it is likely that the objectives will not be met.
The binding of ligand to these targets can be substantially distinct from other target types since such metal ions often coordinate ligand polar atoms, which may help to place and orient the ligand correctly in the binding sites.
In vitro binding of BORIS isoforms to DNA targets can be methylation-sensitive and depends on the number and specific composition of zinc fingers.
We have shown that highly stable binding proteins for a wide spectrum of targets can be generated through mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus.
Only HNE-adducted proteins are biotinylated, so these targets can be purified by binding to streptavidin-coated beads, followed by elution from the beads with UV light.
Development of transient drugs, defined by their binding to target, can be based on high-off-rates, multivalent approaches or multiple targets.
Based on the results of the in vitro experiments the hypothesis of a specific binding to the target can be generated.
By selection with the target of interest, a phage pool that has increasing specific binding ability to the target can be obtained efficiently.
Biomolecular receptors such as proteins or nucleic acids that shift between two or more conformations upon binding to a specific target can be used to build robust, sensitive, and specific sensors.
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