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We observed that some of the regions of the protein structure that exhibit such fluctuations coincide with the protein's binding surfaces with different substrate like GTPase effector domain (GED) of dynamin, SUMO binding motifs (SBM), E1 (activating enzyme, SAE1/SAE2) and E2 (conjugating enzyme, UBC9) enzymes of sumoylation machinery, reported earlier.
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It is known that regions directly involved in biochemical functions, such as binding surfaces, experience different selection pressure from other regions on the surface of proteins [50].
Each of Glo's RNA-binding domains therefore contains two distinct binding surfaces that interact with different types of RNA target sequence.
Two types of surfaces with different polymer chains binding to the substrate were prepared and its ability to reduce the protein adsorption was compared.
We decorated the surface with different TM atoms with different binding energy strengths in order to enrich both adsorption as well as desorption of hydrogen at ambient conditions.
For example, changes in the relative orientation of protein domains make it possible to create distinct binding surfaces for intermolecular interactions with many different binding partners.
Integrins are known to participate in cell adhesion, and work as receptors in cell-surface mediated signalling, through binding with different ligands in focal adhesions [ 8- 10].
A total of 4 different binding surfaces or flow channels were prepared on each chip.
This nucleic-acid-binding surface is entirely different from that of the SRm160 PWI domain.
HOTTIP lncRNA binding surface overlaps with the RbBP5 binding surface on WDR5.
The protein-binding surfaces of ETS factors are different, and different ETS factors associate with different other TFs to bind distinct composite sites (Verger and Duterque-Coquillaud, 2002).
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