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Our finding is consistent with simulation studies for various antifreeze proteins where hydrophobic hydration was found on ice binding surface but not on non-ice binding surface [11], [13], [14], [16].
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This conformational change not only extends a cationic membrane-binding surface, but also exposes hydrophobic and electrostatic protein interacting surfaces for polymerization.
This hydrophobic hydration found on the ice binding surface is obviously not evolved for enhancing ice binding affinity.
Based on the data presented in this manuscript, we now suggest that the interaction between NOXO1 and NOXA1 relies solely on this additional binding surface and does not include the proline-rich region.
The specific residues and chemical details of the binding surfaces are not known.
Secondly, the model shows how buried sequences within the core propeller blades may potentially be exposed by extrusion to provide binding surfaces that are not exposed in the monomeric state.
Again, no appreciable transthiolation defects were observed for the mutants, supporting the notion that the ubiquitin-binding surface is not involved in the upstream steps of the enzymatic cascade.
(B ) Serum survival of E. coli DH5α (which does not express a CFH-binding surface protein) is not altered by pre-incubation with CFHR3.
The DH domain binding surface of SH3(E) does not overlap with the PxxP-binding groove (figure 8).
Our crystal structure indicates that the DH domain binding surface in SH3(E) does not overlap with the proline-peptide (PxxP) binding groove.
Together, these data indicate that the parkin Ubl domain binding surface area for ataxin-3 does not increase as a function of the number of UIM regions, as expected if simultaneous or cooperative binding of multiple UIMs were occurring.
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