It was proposed that residues from HLEs could serve for binding and recognition of RNA substrates, rather than for catalysis.
Thus, it was proposed that LF could be sensed by NLRP1 through the presence of cleaved host substrates rather than direct binding (Fink et al, 2008).
These observations, as well as comparison to structures of other kinases bound to substrates, indicate that the inhibitory peptides act as true pseudo-substrates that mimic binding to substrate, rather than stabilizing a distinct catalytically inactive conformation of GSK-3.
On the basis of these data, we propose that structural maturation of the client protein substrate, rather than ATP binding or hydrolysis, serves as the primary signal for dissociation of GRP94-client protein complexes.
The notion that even Siz1 mutants defective in DNA binding preferentially modify PCNA when the clamp encircles DNA raised the question of whether stimulation of the reaction could be ascribed primarily to DNA-induced changes in the substrate rather than a proximity effect mediated by the binding of substrate and E3 to a common stretch of DNA.
The conserved cysteine residues, located in the N and C termini, are likely to be involved in substrate binding rather than substrate transport.
R45A lost transport activity but retained a FRET change response after the addition of substrate rather than showing a quenching response, indicating an overall conformational change due to binding of nitrate in the absence of a functional transport cycle.
He's looking forward to yet more development, "when we can have the thin films of today on a flexible substrate" rather than the present glass substrates, so that the TVs can be rolled up and tucked into a backpack.
Using purified KDM5A, we demonstrate that this allosteric enhancement is caused by improved substrate binding rather than enhanced catalytic turnover.
