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However, new binding studies with the mammalian αβγ complex confirm that only two bind reversibly (Xiao et al., 2007).
Also, binding studies with the corresponding natGa- and natLu-labelled compounds revealed no difference to the "metal-free" peptide.
In addition product inhibition was assessed by binding studies with the nucleoside monophosphate derivatives and thermodynamic parameters were established.
Both ERα and ERß have been cloned and used in in vitro binding studies with the BNO 1055 CR extract [20].
In receptor binding studies with the radioligand [3H]-deltorphine II only ent-3 showed considerable δ receptor affinity (Ki = 740 nM).
We also performed metal-ion binding studies with the isolated EF-hand used in the computational work to examine the ability of Al3+ and comparative metals to bind the peptide.
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Using competition-binding studies with the protein natural ligand T4, we have identified one prenylated xanthone with a very strong affinity to transthyretin.
The βAR subtype was determined by competition-binding studies with the β1-selective adrenergic antagonist CGP20712A and the β2-selective adrenergic antagonist ICI118551.
The inhibition and binding studies with fosmidomycin reinforce the importance of the loop as a determinant in ligand association.
For the cyanide binding studies with ferric NwCld, the conventional stopped-flow mode was used and the increase in absorbance at 420 nm was monitored.
Next, we performed competitive binding studies with AtraPBP1 incubated with the three ligands at the same concentration.
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