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Lipid concentrations used in the binding studies were selected to give binding responses of 30-500 resonance units.
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Twenty-four studies were selected.
Forty studies were selected.
Twelve validation studies were selected.
To obtain a CSA-binding phenotype, FCR3-CSA and 3D7-CSA IE used in this study were selected on the trophoblastic BeWo cell line as described recently [15].
NF-κB sites for study were selected by the CLOVER algorithm of Motifviz (http://biowulf.bu.edu/MotifViz/), which uses the JASPAR database (http://jaspar.genereg.net/) for its binding matrices.
Binding studies were also conducted for selected mutants.
UTP, GTP, and AMP binding studies were carried out for selected aptamers identically to the ATP studies to analyze the impact on specificity and selectivity.
Saturation binding studies were carried out to analyse the in vitro binding properties of the diabody.
Residues defining the binding sites were selected for site-directed mutagenesis.
Representative structures for each ligand were analyzed and binding modes were selected according to the mutagenesis data.
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