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Although molecular modeling and DNA binding studies were reported, the detailed mode of action and animal data were not available.
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To provide a more concrete support for such a model, ACTD equilibrium binding studies were carried out and the results are reported herein on oligomers of sequence motifs d TGTCTnG) and d TGTnGTC).
The lack of activity with I− likely results from a steric inability of this larger ion to bind the correct coordination site, although as yet no I− binding studies have been reported.
Strangely enough, no binding studies have been reported for primer-template DNA (p/t-DNA) even though PrimPol performs DNA synthesis on these types of substrates.
At the functional level, biphasic Ca2+-activating profiles like the one observed in our [H]ryanodine-binding studies have been reported previously in RyR3 [ 13] and RyR2 [ 40, 41] and are consistent with our hypothesis that activation of RyRs may involve at least two moderately co-operative Ca2+-activation sites [ 13].
Though detailed binding studies have not been reported, evidence from numerous studies suggests that ε-toxin binds to a specific receptor.
The integrin αVβ3 receptor binding study was performed using a previously reported procedure with a slight modification [25].
In contrast, Kd values >2.5 µM in the direct binding affinity studies have been reported for the same proteins [17].
Five SAEs were reported in this study.
The sequences of the peptides used in this study, along with their respective binding affinities are reported in Table 2.
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